On the other hand, the tumor uptake of [64Cu]Cu-NOTA-EV-F(ab′)2 peaked at 4 h after injection (Fig. 4B), with values of 11.37 ± 0.62, 8.70 ± 0.43, and 5.20 ± 0.49 %ID/g in bladder tumors with high, intermediate, and low nectin-4 expression, respectively. This evidence concerns the gene NECTIN4 and neoplasm.