We next performed CRISPR/Cas9 editing in 3 primary human AML samples with NPM1c mutations using the same GAB2 and AAVS1 guides, and then transplanted these cells into NOD Scid Gamma mice expressing human SCF,GM-CSF, and IL-3 (NSG-SGM3 mice) (47). Here, GAB2 is linked to acute myeloid leukemia.