GAB2 and acute myeloid leukemia: To determine whether Gab2 is required for the maintenance of fully transformed AML cells with +7, we utilized CRISPR/Cas9-mediated genome editing to inactivate Gab2 (or Rosa26, as a negative control) in 5 independent, fully transformed murine AMLs (mAMLs) from this model, and then transplanted these cells into recipients to determine their fate in vivo (Figure 7A).