Furthermore, genes such as Ptk7 (overexpressed in colon carcinoma cells), Mex3a (a marker of drug-tolerant persister CRC cells) and Yap (involved in 5-FU resistance in CRC cell lines), were upregulated following regorafenib treatment alone but returned to baseline levels whith combination treatment (Figure 3C) (Jin et al., 2024; Álvarez-Varela et al., 2022; Xu et al., 2024). This evidence concerns the gene PTK7 and colon carcinoma.