TFs exhibited antiproliferative, pro‐apoptotic, antimigrative, and tumor‐inhibitory effects on B16F10 melanoma cells in a dose‐dependent mannerTFs upregulate the mRNA expressions of pro‐apoptotic genes and protein expressions of apoptosis‐related p53 and JNK signaling molecules and downregulate the protein expressions of proliferation‐related MEK/ERK and PI3K/AKT signaling molecules as well as the expressions of MMP2 and MMP9. The gene discussed is MAPK8; the disease is melanoma.