TF showed anti‐proliferative and pro‐apoptotic effects on A375 human melanoma cells in concentration‐dependent manner via P53 and JNK pathwaysTF markedly stimulated proteins associated with the P53 pathway (ATM, CHK1/2, P53, and CASP8/3) and those linked to the JNK pathway (ASK1, JNK, and C‐JUN) via phosphorylation and cleavage, leading to the activation of pro‐apoptotic factors (PARP, BAX, BIM, PUMA, and P53). Here, MAP3K5 is linked to melanoma.