Regarding the co-infection of cells by E. coli and S. aureus, the unique genes of ES2 are DZIP1 and SMTNL2; the unique genes of ES3 are ALDH4A1, DNASE1L1, FAM227A, and KAT2B; and ES1 has 143 unique genes (Supplementary Figure S3). The gene discussed is FAM227A; the disease is coinfection.