However, the percentage of high PGK1 expression levels in the cancer tissues of patients with low differentiation, TNM stages III–IV, lymph node metastasis, and tumor diameter ≥1.0 cm was greater than that of patients with high or moderate differentiation, TNM stages I–II, no lymph node metastasis, and tumor diameter <1.0 cm (P < 0.05; Table 3). This evidence concerns the gene PGK1 and metastatic malignant neoplasm in the lymph nodes.