The transfection cytology methods were used to detect autoimmune encephalitis antibodies (NMDAR, AMPAR1, AMPAR2, LGI1, CASPR2, GABABR, DPPX, IgLON5, GlyRα1, GABAARα1, GABAARβ3, mGluR1, mGluR5, D2R, Neurexin-3α, GAD65, KLHL11, gAChR, AQP4, MOG, GFAP) in CSF and serum. The gene discussed is GAD2; the disease is autoimmune encephalitis.