On theone hand, a higher degree of α-synuclein purification increases the assayduration; on the other hand, it significantly reduces the background noise andallows for extended incubation times, which are essential for the reliabledetection of synucleinopathies such as MSA, which are known for their weakseeding activity in the RT-QuIC assay [19]. This evidence concerns the gene SNCA and synucleinopathy.