1) TILs cultured from tumor fragments (22 tumors). Culturing 1–4 tumor fragments in 2 mL of the TIL growth medium containing IL-2. Haft of the growth medium was replaced with fresh one every 2–3 days. 2) TILs from enzymatically digested tumor fragments (12 tumors). Solution for enzymatic digestion: collagenase D 10 mg/mL, DNase I 3 mg/mL. 30-min incubation on a GentleMACS dissociator. 3) TILs from enzymatically digested tumor fragments with additional positive CD3 selection using Dynabeads magnetic beads (25 tumors). Here, IL2 is linked to neoplasm.