1) TILs cultured from tumor fragments. Growth medium: RPMI 1640, 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mmol/L L-glutamine, 10% human serum, IL-2 (6,000 IU/mL). 2) TILs from enzymatically digested tumor fragments. Solution for enzymatic digestion: collagenase, hyaluronidase, and DNase in RPMI 1640. 18-hr incubation of the fragments on an orbital shaker. Culturing the resulting cell suspension in the TIL growth medium. The gene discussed is IL2; the disease is neoplasm.