1) TILs cultured from tumor fragments. Culturing one tumor fragment per well of a 24-well plate in the growth medium containing IL-2 (6,000 IU/mL). 2) TILs from enzymatically digested tumor fragments. Solution for enzymatic digestion: DNase IV (30,000 U/L), hyaluronidase V (100 mg/L), collagenase IV (1,000 mg/L), gentamicin (500 mg/L), penicillin-streptomycin (5,000 U/mL), L-glutamine (292 mg/L), amphotericin B (62.5 μg/L). Culturing cell suspension in the growth medium containing IL-2 (6,000 IU/mL). The gene discussed is IL2; the disease is neoplasm.