For instance, in microsatellite instability-high (MSI-H) CRC, three-dimensional reconstruction models from Visium data show that activated (GZMB+, and IFN-γ+) CD8+ T cells tend to cluster at the tumor-stroma interface, forming tight spatial interaction clusters (average distance <20 μm) with CXCL9-expressing myeloid cells. Here, CD8A is linked to neoplasm.