By combining scRNA‐seq and stRNA‐seq technologies, we revealed stage‐specific gene expression characteristics of T cells, uncovered pivotal exhaustion‐associated markers in CD8+ T cell populations, including TNFRSF18 and CXCL13,9, 12, 13, 14, 15 and explored the communication networks between immune cells and tumour cells.16 This evidence concerns the gene CD8A and neoplasm.