In order to deeply explore the material basis of the anti-AD efficacy of HLJDD, we performed UPLC-MS/MS analysis with gradient elution using 0.1% formic acid aqueous solution-0.1% formic acid acetonitrile solution as the mobile phases, and full scans of the extract of HLJDD, brain tissue of APP/PS1 mice, and plasma of APP/PS1 mice were carried out in both the positive and negative ionization modes, and the sample base-peak ion the total ion current (TIC) chromatograms are shown in Fig. 1A–F. The gene discussed is APP; the disease is Alzheimer disease.