Given the limitation that BALB/c nude mice are immunocompromised, we further examined the role of Nuak2 using a piggyBac in utero electroporation (PB-IUE) model of malignant glioma (Zhang and Bordey, 2023; Chen and LoTurco, 2012; Glasgow et al, 2014). Here, NUAK2 is linked to malignant glioma.