We perfused 3D-BBB microvessels with highly synchronized trophozoite (26–34 h post infection) or schizont (38–46 h post infection) stages of P. falciparum HB3var03, a parasite line expressing a dual EPCR-ICAM-1 binding PfEMP1 (Supplementary Fig. 5a). This evidence concerns the gene ICAM1 and infection.