HECTD4 and neoplasm: In a control experiment, a 1:1 mixture of GFP- and mCherry-tagged MDA-MB-231 cells (GFP-shControl; HECTD4-WT vs. GFP-mCherry-shControl; HECTD4-WT) (n = 4-5 per group) shows persistence of the 1:1 ratio in primary tumor (orthotopic mammary fat pad), comparable with the ratio on day 0 input cell population, as measured by FACS analysis.