NRL and retinoblastoma: To assess endogenous Tr-NRL expression, we performed immunoblot analysis of CHLA-VC-RB31 retinoblastoma cells (Stachelek et al., 2023), which were predicted to express FL-NRL and Tr-NRL transcripts in a cone-like 0.73:1 ratio, with an antibody that recognizes both FL-NRL and Tr-NRL proteins (Figure 3—figure supplement 2A and B).