As Dijkstra et al. (2018) have established an organoid model of a tumor-like microenvironment, which is a co-culture system containing IL-2 mediators using anti-CD28 and anti-PD-1(Programmed Death-1,PD-1) antibodies against peripheral blood mononuclear cells (Peripheral blood mononuclear cells (PBMC) were stimulated with anti-CD28 and anti-PD-1(Programmed Death-1,PD-1) antibodies to induce the generation of lymphocytes, which were then co-cultured with interferon-γ (IFN-γ) pretreated monocyte suspensions. The gene discussed is CD28; the disease is neoplasm.