(3)This study primarily focused on miR-27b’s regulation of Mff but did not exclude the influence of other potential targets through genome-wide methods; (4) Although a functional association between Mff and MAVS was observed, the direct molecular interaction mechanism requires further elucidation; (5) Systematic evaluation of dynamic expression changes of miR-27b at different sepsis stages and its temporal relationship with disease progression was not performed. The gene discussed is MAVS; the disease is Sepsis.