PLE, as a traditional Chinese medicine extract, may improve the symptoms of anxiety and irritability as well as hot flashes in model rats by inhibiting the activation of Tac3 neurons, decreasing Tacr3 expression and increasing Cplx1 expression, thus coordinating the response of hypothalamic neural circuits and increasing E2 and 5-HT levels in vivo. Here, CPLX1 is linked to Anxiety.