Within the Pax6+ clusters 0–9, based on marker gene expression and cell cycle phase (Fig. 8D, E), clusters 3, 4, 8 were proliferative (Ki67+) and enriched for the GCP markers Gli1, Atoh1 and Barhl1, indicating highly proliferative GCP-like tumor cells. The gene discussed is BARHL1; the disease is neoplasm.