PTEN and neoplasm: We next performed differential expression analyses between SmoM2 and SmoM2-Ptenfl/fl cells in the GCP-like tumor cell clusters (3, 4, 8) and found 1,011 genes were significantly upregulated and 3,710 were downregulated in the cells lacking Pten compared to SmoM2 cells (adjusted p-value≤0.05, Fig. 9A, Table S3).