SPI1 and cancer: Intriguingly, integrating results from genome-scale CRISPR-Cas9-based fitness screens performed across >1000 cancer cell lines, we find that AML cell lines exhibit among the highest co-dependency scores on both PU.1 and SMARCD2, suggesting the possibility that the interaction between PU.1 and SMARCD2 is necessary for oncogenic transcriptional functions in these cells (Fig. 4A, S7A).