TNFRSF1A and metabolic dysfunction-associated steatotic liver disease: To test the hypothesis that TNFα was critical for the development of NAFLD caused by choline-deficient diet, wild type and TNFR1-/- mice were given standard AIN-76 (fully supplemented) diet or L-amino acid defined choline-deficient AIN-76 diet for 4 and 10 weeks.