To further identify core biomarkers and establish an accurate diagnostic model, we identified differentially expressed genes in Cluster2 between stage I-II and N0M1 CRC (Wilcoxon test, p<0.05) (Figure 2G), after eliminating redundant genes using Lasso regression (Figures 2H, I), 9 genes were selected as LIMGs (ACTG2, HSPH1, ITGA11, LAMA5, HSPB1, THBS1, SORBS1, POSTN, NID1). Here, HSPB1 is linked to colorectal carcinoma.