Since there was no significant difference expression in RIMS3 and EXPH5, we only studied LY96 and TMEM140 further, in our study, LY96 mainly distributed in monocytes, DC and T lymphocytes and TMEM140 was mainly expressed in T lymphocytes cluster in SLE samples, and The expression level of LY96 and TMEM140 were both elevated Compared with control group, which is consistent with our research above. The gene discussed is TMEM140; the disease is systemic lupus erythematosus.