Western blot and qPCR analyses also demonstrated downregulated expression of both SOX17 and Nestin, along with upregulated p16INK4a expression in pulmonary microvascular ECs from rats with PAH induced by monocrotaline (MCT) as compared with control ECs (Fig. 3F–I). The gene discussed is CDKN2A; the disease is pulmonary arterial hypertension.