METTL3 and cancer: To simulate renal tubular injury in cancer patients undergoing chemotherapy, we established in vitro models by exposing BUMPT cells to low-dose CDDP for 5 hours daily over a consecutive 72-hour period, as detailed in Fig. 1A. Immunoblot analysis demonstrated that CDDP induced the expression of FN, collagen I, collagen III, and Mettl3 in a dose-dependent manner at concentrations of 1 μM, 2 μM, and 5 μM (Fig. 1B & C).