Similar to 2D results, despite a difference in the treatment concentration, cleaved caspase 8 and PARP expression markedly increased, whereas Mcl‐1 expression decreased upon PYR administration, indicating that PYR may induce Mcl‐1‐mediated and caspase‐dependent apoptosis in cancer cell spheroids (Figure 5B). The gene discussed is CASP8; the disease is cancer.