Finally, in interesting parallel, knockdown of DNMT3A also would prevent hypoxia-induced EMT, transcriptional activation of TWIST1 gene, and global genomic 5mC demethylation in the breast cancer cells (MCF-7) and the hepatocellular carcinoma cells (Hep G2) (Figs. 4 and S4). Here, DNMT3A is linked to breast cancer.