Thus, DNMT3A appears to be a major player for the hypoxia-induced EMT process of the colon cancer cells via DNA demethylation, by its DNA demethylation activity, of the SW480 genome including the promoters of essential EMT associated genes like TWIST1 and SNAIL1. It should be mentioned here that the hypoxia condition is known before to induce genomic DNA demethylation globally in human colon cancer cell lines HCT116, DLD-1 and SW480, melanoma cell line WM115, and dermal fibroblast [65]. This evidence concerns the gene SNAI1 and malignant colon neoplasm.