Analysis of the public data set GSE206768 containing PMF samples with known MF grade revealed a significant upregulation of 42 genes (p < 0.05) including e.g., IL-8 and TGF-ß in patients with MF-3 versus MF-0 (Supplementary Fig. S2A) and genes related to IFN-γ response in PMF samples with high myelofibrosis (MF-3) compared to low myelofibrosis (MF-0) (Supplementary Fig. S2B, C). The gene discussed is CXCL8; the disease is myelofibrosis.