To investigate the underlying mechanism of RUNX1’s indispensable role in CBFB-MYH11–induced leukemia (18), we conducted an integrated analysis of Cbfb-MYH11 and two Cbfb-MYH11 mutants, Cbfb-MYH11-ΔHABD and Cbfb-MYH11-mDE (as illustrated in Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/JCI192923DS1), regarding their effects on RUNX1 function. The gene discussed is CBFB; the disease is leukemia.