To clarify the role of NR4A3 in BLCA anoikis resistance and metastasis, adenovirus expression vectors were used to mediate NR4A3 overexpression in T24 and 5637 cells, the efficiency of infection was validated by the GFP fluorescence in cells and the RT-qPCR, the mRNA expression of NR4A3 was elevated by more than twofold (Figure 2a). This evidence concerns the gene NR4A3 and infection.