The results were refined to analyze the recruitment of different immune cells in step 4 by the GPR-TME subgroups, which revealed that the GPRlow/TMEhigh subgroup had a greater ability to recruit immune cells, especially CD8+ T cells, dendritic cells, macrophages, NK cells, monocytes, and Th 1 cells, which might have greater anti-cancer activity in the cycle of immune cell functioning. The gene discussed is ALDH18A1; the disease is cancer.