We conjointly analyzed the single‐cell transcriptome and m5C epitranscriptome, and the results showed that most of the significantly changed m5C regulators, including ten‐eleven translocation 2 (TET2), NOP2/Sun RNA methyltransferase 2 (NSUN2), NOP2/Sun RNA methyltransferase 3, NSUN4, tRNA aspartate methyltransferase 1, DNA methyltransferase 3β, and NOP2/Sun RNA methyltransferase 7 (NSUN7), were upregulated in SLE patients, especially m5C eraser TET2 and writer NSUN4 (Figure 5A). This evidence concerns the gene NSUN7 and systemic lupus erythematosus.