Furthermore, TSSC3 intervention effectively reduced PI3K-P85 and AKT levels in MyoFb, demonstrating that downregulating the PI3K/Akt pathway suppresses both anoikis resistance and pro-fibrotic capacity of renal-derived MyoFb, thereby attenuating renal fibrosis (Liu et al., 2022). Here, AKT1 is linked to renal fibrosis.