By using CT26 colorectal cancer bilateral tumor‐bearing mice as an experimental animal model, it was found that compared to RT treatment alone, the expression of MHC‐I molecule on M2 TAMs was upregulated by 23.6% and the population of activated IFN‐γ+CD8+ T cells improved by 17.4% when Ft‐E64/Hf@Lipo was combined with RT, significantly improved the antigen presentation process and T cell responses. This evidence concerns the gene IFNG and colorectal cancer.