In recent years, studies have reported that RT can lead to upregulation of PD‐L1 expression in tumor cells, which greatly limits the effectiveness of radio‐immunotherapy, and accumulating evidence suggests that RT synergizes with anti‐PD‐L1 to effectively augment the antitumor responses.[15] Here, through flow cytometry analysis, we also observed that the expression of PD‐L1 on tumor cells was upregulated 1.9 times after RT (Figure S18, Supporting Information). The gene discussed is CD274; the disease is neoplasm.