The T cell-mediated tumor cell killing assay showed that compared with 231M3PDL1, the apoptosis rate of 231M3N219Q was significantly inhibited (Fig. 6E), accompanied by the decrease of effector cytokines IFN-γ and TNF-α secreted by CD8+ T cells in co-culture system (Fig. 6F). Here, CD8A is linked to neoplasm.