Next, in order to further investigate the possible mechanisms of 25R-inokosterone, we performed KEGG enrichment analysis on the hub gene, and the results showed that it was mainly concentrated in the PI3K-Akt signaling pathway, mTOR signaling pathway, prolactin signaling pathway, aldosterone-regulated sodium reabsorption, chemical carcinogenesis (receptor activation), and type II diabetes mellitus. Here, AKT1 is linked to type 2 diabetes mellitus.