In a rat model of nonalcoholic fatty liver disease induced by a methionine-choline-deficient diet, Jian-Pi Huo-Xue significantly reduced serum alanine aminotransferase and aspartate aminotransferase levels, decreased the accumulation of triglycerides and total cholesterol in the liver, and improved liver inflammation.[41] Another study investigated the therapeutic effects of a similar Jianpi Jiedu Recipe on colon cancer both in vitro and in vivo. This evidence concerns the gene GPT and colonic neoplasm.