AQP5 and colorectal cancer: Therefore, in this study, we transfected AQP5 overexpression plasmid into human colorectal cancer cell lines RKO and HCT116 to explore the effect of AQP5 combined with 5-FU on the proliferation and apoptosis of colorectal cancer cells and the underlying mechanism, and verified the results of in vitro experiments using SPF nude mice.