Crucially, AQP5 overexpression counteracted 5-FU’s therapeutic effects: compared with the group treated with 5-FU alone, the expression level of p-NF-κB was up-regulated and the number of apoptosis was reduced in the group treated with AQP5 overexpression and 5-FU alone, suggesting that AQP5 could restore the inhibitory effect of 5-FU on the malignant progression of colorectal cancer. This evidence concerns the gene NFKB1 and colorectal cancer.