In order to assess the relative selectivity of POLE inhibition in CIC::DUX4 sarcomas, we tested the impact of POLE expression on non-CIC::DXU4 sarcoma subtypes, including rhabdomyosarcoma cells (Rh30, RD) and Ewing sarcoma (CHLA10, A673). Here, POLE is linked to Ewing sarcoma.