Using genetic silencing of POLE in CIC::DUX4 sarcoma cell lines (Supplementary Fig. 5), we observed that POLE suppression significantly reduced the growth and/or viability of both NCC_CDS1_X1_C1 and NCC_CDS2_C1 cells, as assessed by Cell-Titer-Glo, trypan blue staining, and crystal violet assays (Fig. 2D, E). The gene discussed is POLE; the disease is sarcoma.