Immunofluorescence analysis demonstrated that 28 days post-lymphatic injury, mice with lymphedema fed the HSFD exhibited significantly higher levels of 8-OHdG, with a Pearson correlation of 0.54 with LYVE-1 positive cells, compared to the CD- (Pearson correlation: 0.21) and the HFD- (Pearson correlation: 0.31) groups (Fig. 4A,B; Appendix Fig. S12). This evidence concerns the gene LYVE1 and lymphedema.