We first examined H3K27me3 expression in LNCaP-EnzR cells and other prostate cancer cell lines and found that H3K27me3 was much lower in LNCaP-EnzR cells than in other late-stage prostate cancer cells (Fig. 4n; Supplementary Fig. S22a, b), suggesting that the decrease in H3K27me3 might be connected to the increased expression of TDO2 at the CRPC stage. This evidence concerns the gene TDO2 and Familial prostate cancer.