LYVE1 and neoplasm: Regarding macrophages, the immunofluorescence results showed a significant increase in the number of M2-type macrophages (CD68+ LYVE1+ and CD68+ CD163+) in the tumor tissues of the nLCR group (Fig. 7D, E), validating the enrichment of immunosuppressive macrophage subclusters (such as macrophages_LYVE1) in poor-response tissues found in the scRNA-seq analysis.