Our test system was a 20mer ASO that targets the gold-standardknockdown target metastasis-associated lung adenocarcinoma transcript1 (MALAT1), a nuclear-enriched long noncoding RNA(lncRNA).MALAT1 playskey roles in gene regulation and metastasis in cancer, and is primarilyretained within the nucleus., Implementing the currentstate-of-the-art in ASO design, this MALAT1-ASO had a gapmer design,containing a fully PS backbone with terminal wings of five 2′-methoxy-ethyl(MOE) sugar modifications (Figure a,b). The gene discussed is MALAT1; the disease is cancer.