The average percentage of methylation for unmethylated and methylated DNA was 1,85% (unmethylated) and 97.71% (methylated) for MGMT, 6% (unmethylated) and 72.3% (methylated) for CDKN2A and 1.5% (unmethylated) and 88.75% (methylated) for PROM1. Last CRC biomarker, ADAMTS16, showed a certain degree of hypermethylation in its selected region according to pyrosequencing analysis. The gene discussed is MGMT; the disease is colorectal carcinoma.