YAP1 and myocardial infarction: To investigate whether PSAT1 is upstream of YAP1-β-catenin nuclear interaction and whether YAP1 inhibition affects PSAT1-induced CM cell cycle in the heart post-MI, we retro-orbitally injected AAV9-GFP, AAV9-shRNA-scramble or AAV9-shRNA-YAP1 seven days before LAD ligation and PSAT1 modRNA delivery to infarcted myocardium (Figure 7A-E).