These cells predominantly exhibited two distinct spatial distribution patterns: one group, identified as astrocyte subgroup 6, was located in the infarct area of the stroke and was primarily characterized by high expression of Apoe, Clu, and Cd81; the other group was located in the peri-infarct region and involved astrocyte subgroups 3, 5, 7, and 9, with subgroup 5 being particularly characterized by high expression of Syne1, Lars2, and Macf1. The gene discussed is CD81; the disease is stroke disorder.