BAG2 and gastric cancer: To verify this hypothesis, we further investigated the expression levels of BAG2 mRNA and protein in the normal gastric mucosa cell line GES and various gastric cancer cell lines, including MKN-45, HGC-27, SNU-216, AGS, and NCI-N87, using quantitative real-time polymerase chain reaction (qRT-PCR) and immunoblotting (Supplementary Figures 1A, B).