To further investigate the potential roles of the eight key genes (LIMS2, TP53INP2, IRAK3, STX2, CYP27A1, IL11RA, KCNMB1, and PDLIM7) in bladder tumorigenesis, we performed qRT-PCR to quantify their expression levels in the bladder normal cell line SV and bladder cancer cell lines 5637, T24, and HT1376. The gene discussed is LIMS2; the disease is urinary bladder carcinoma.