UM-UC5 and UM-UC9 bladder cancer cells and BT-20 and MDA-231 breast cancer cells were treated with 10 uM ambrosin over a 48 h time course before cellular proteins were isolated for Western blotting and probed with anti-PARP and anti-GAPDH antibodies. The gene discussed is GAPDH; the disease is urinary bladder carcinoma.